An NTUA Diploma Thesis titled “The effect of fatigue on driver behavior in rural environment using driver simulator” was recently presented by Georgios Tziouvaras. To achieve this objective, a driving simulator experiment was carried out and data from 35 participants were collected. Linear and binomial logistic regression models were developed. Results indicated that fatigue driving increases the average speed and reaction time of drivers, reduces their distance from the vehicle ahead and increases crash probability. It was also revealed that under conditions of high traffic load, lower average driver speeds, reduced reaction times and increased crash probability were observed.